Process for the detection and assay by erythroadsorption

Details Process for the detection and assay by erythroadsorption Process for the detection and assay by erythroadsorption

1985-12-11

1988-03-08

Moskowitz, Margaret

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

436520, 436521, 436522, 436531, 436809, 436819, 436825, 436827, 435 7, 435188, 435 6, G01N 33566, G01N 33555

Patent

active

047299614

ABSTRACT:
A process for the detection and assay of a biological substance by erythroadsorption by immobilizing a substance having a binding affinity for the biological substance to be assayed; incubating the immobilized substance with a liquid medium containing the biological substance to be assayed, forming a fixed substance; incubating the fixed substance with a coupling product comprising a specific ligand and a second ligand which binds with erythrocytes; adding erythrocytes; and determining the amount of erythrocytes bound to the coupling product. The determination of the amount of bound erythrocytes can be made visually with the naked eye or determined by lysis of the erythrocytes bound to the coupling product and quantitatively measuring the released hemoglobin. The specific ligand can be an antibody or an antigen and the second ligand capable of coupling erythrocytes can, for example, be red blood cell antibodies.

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