Production of recombinant human interferon-beta2

Details Production of recombinant human interferon-beta2 Production of recombinant human interferon-beta2

1992-05-15

1996-04-23

Martinell, James

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6951, 435 6952, C12N 1522, C12N 1524

Patent

active

055104720

ABSTRACT:
Human interferon-beta.sub.2.sbsb.A and interferon-beta.sub.2.sbsb.B are produced in purified form by recombinant DNA techniques. Two separate human genes have been identified which code for the production of IFN-.beta..sub.2.sbsb.A and IFN-.beta..sub.2.sbsb.B, respectively. The sequence of IFN-.beta..sub.2.sbsb.A cDNA is established. These genes and cDNA have been cloned into mammalian cells with an SV40 early promoter sequence and such genomic clones are capable of producing IFN-.beta..sub.2.sbsb.A and IFN-.beta..sub.2.sbsb.B. The antiviral activity of such recombinant IFN-.beta..sub.2.sbsb.A and IFN-.beta..sub.2.sbsb.B is demonstrated as well as other biological activity identifying them as human interferons.

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