Fluoroimmunoassaying

Details Fluoroimmunoassaying Fluoroimmunoassaying

1983-02-28

1986-03-18

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

436526, 436528, 436529, 436530, 436531, 436537, 436800, 436807, 436810, G01N 5300, G01N 33553, G01N 33544, G01N 33542

Patent

active

045769125

ABSTRACT:
A method of composition is featured for performing a fluoroimmunoassay of a biological fluid sample for an immunological reactant. A conjugate of a carrier labelled with fluorophores and coupled to an immunological reactant is mixed with the sample and a known quantity of binding agent. After equilibrium is accomplished, the bound and unbound portions are separated. The carrier in a selected portion is chemically treated to liberate the fluorophores. The fluorescent level is then measured and compared with a standard.

REFERENCES:
patent: 4000252 (1976-12-01), Kosak
patent: 4039652 (1977-08-01), Adams
patent: 4108972 (1978-08-01), Dreyer
patent: 4133873 (1979-01-01), Nollor
patent: 4231999 (1980-11-01), Carlsson et al.
patent: 4272506 (1981-06-01), Schwarzberg
patent: 4279992 (1981-07-01), Boguslaski et al.
patent: 4320109 (1982-03-01), Wolf et al.

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