Micropipette adaptor with temperature control for PCR amplificat

Optics: measuring and testing – By dispersed light spectroscopy – Utilizing a spectrometer

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356244, 356436, G01N 2101, G01N 2131

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active

052413632

ABSTRACT:
A temperature-controlled micropipette adaptor includes a metal base sandwiched between two plastic layers. The metal base has an orifice to hold a micropipette. The plastic layers hold lenses in alignment for spectrophotometric measurements of a sample contained in a micropipette inserted into the orifice. A resistive heater wire or thermoelectric heater/cooler is held between the metal base and the plastic layer to transfer heat from the heater wire or thermoelectric device to the metal base and thus to the micropipette sample. A thermocouple is attached to the metal layer to monitor temperature changes. A feedback control system is coupled to the device for monitoring and programmably controlling changes in temperature of the heated sample over time. As desired, a microprocessor can be electrically connected between the heater wire or thermoelectric device and the output signal of the spectrophotometer to selectively energize the heater wire thermoelectric device in response to the output signal of the spectrophotometer.

REFERENCES:
patent: 3877817 (1975-04-01), Ralston
patent: 4006990 (1977-02-01), Munk
patent: 4440497 (1984-04-01), Carey et al.
patent: 4935875 (1990-06-01), Shah et al.
patent: 4991958 (1991-02-01), Garner
patent: 5092674 (1992-03-01), Garner

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