Column method of immunoassay employing an immobilized binding pa

Details Column method of immunoassay employing an immobilized binding pa Column method of immunoassay employing an immobilized binding pa

1973-10-11

1977-08-02

Padgett, Benjamin R.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 232306, 424 12, 250303, 424 15, G01N 3300, G01N 3316, G21H 502

Patent

active

040396525

ABSTRACT:
An improved method is disclosed for determining substances in a fluid sample having a mutual specific binding affinity, such as antigens and antibodies, said method utilizing a device comprising a column containing an insoluble porous matrix with which specific binding partners to the substance being determined are immobilized, preferably by chemically coupling them to the matrix material. The method comprises allowing a fluid sample containing the substance to be determined, a reference sample containing a labeled form of either the substance under determination or a specific binding partner, and an eluting liquid to flow through the device, followed by a determination of the relative amount of the labeled component retained in or eluted from the column. Comparison to standard values affords a reading of the concentration or absolute amount of the substance being determined in the fluid sample.

REFERENCES:
patent: 3284434 (1966-11-01), Sutherland
patent: 3555143 (1967-06-01), Axen et al.
patent: 3592888 (1971-07-01), Wolf
patent: 3652761 (1972-03-01), Weetall
patent: 3720760 (1973-03-01), Bennich et al.
patent: 3791932 (1974-02-01), Schuurs et al.
patent: 3843444 (1974-10-01), Likhite
patent: 3961894 (1976-06-01), Gordon et al.
Miles et al., Nature, vol. 219, July 13, 1968, pp. 186-189.
Goodfriend et al, Immunochemistry, vol. 6, May 1969, pp. 481-484.
Catt et al., Biochem. J., vol. 100, 1966, pp. 31c-33c.
Kirkham and Hunter, Ed., Radioimmunoassay Methods, 1970, pp. 405-412.

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