System and method for quantifying macrophage phagocytosis by com

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3649222, 250572, G06F 1500, G06F 1542

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051629909

ABSTRACT:
The present invention is embodied in a method and algorithm for rapidly quantifying phagocytic functions using computer image analysis (CIA) of video light microscopic images. The method and algorithm involve sequential acquisition of bright field or phase contrast and epi-fluorescence video microscopic images of respective field, addition of the images, decision making, object referencing, morphological feature extraction, arithmetic operations, and statistical analysis. This invention provides significantly faster phagocytic functions analysis than manual microscopic examination and more detailed quantitative morphological data than flow cytometery.

REFERENCES:
The IBAS Bag of Tricks, Jul. 1984 (4 pages).
J. Immunological Methods, 64, pp. 71-83 (1983) Elsevier.
J. Histochemistry and Cytochemistry, vol. 33, No. 6, pp. 551-556 (1985).
Methods in Enzymology, vol. 132, pp. 183-192, (1986).
Reviews of Infectious Diseases, vol. 10, No. 1, pp. 92-102, (Jan.-Feb., 1).
Reviews of Infectious Diseases, vol. 11, No. 1, pp. 16-33, (Jan.-Feb., 1988).
Infection and Immunity, pp. 917-919, (Dec., 1986).
Eur. J. Clin. Microbiol., pp. 379-391, (Aug. 1985).

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