Tissue culture technique for asexual plant reproduction and a me

Details Tissue culture technique for asexual plant reproduction and a me Tissue culture technique for asexual plant reproduction and a me

1976-09-27

1977-08-02

Bagwill, Robert E.

Plant husbandry

Process

A01G 3100

Patent

active

040387780

ABSTRACT:
A tissue culture technique and a culture medium used therewith for asexually propagating a plurality of plants of the family Cruciferae are disclosed.
According to one aspect of the invention, an excised portion of a donor plant is placed in a culture medium comprising organic and inorganic salts and a preferable concentration of 1 mg/l of Indoleacetic Acid, 0.5 mg/l of Kinetin and 40 mg/l of Adenine Sulfate. The medium induces the simultaneous initiation of buds and roots by the explant to form a plurality of plantlets which may be transplanted into soil for the timely initiation of food and medicinal crops.
In accordance with a second aspect of the invention, the pre-culture storage of the requisite plant part in a convenient, disease-free, and pest-free manner is disclosed whereby callus from the excised portion of the donor plant is maintained on a first medium for subsequent transfer to the medium described above. The callus is initiated and maintained, for periods in the order of a year or more, on a medium containing organic and inorganic salts and preferable concentrations of 1.86 mg/l of naphthaleneacetic acid and 2.25 mg/l of N.sub.6 -Benzyladenine.

REFERENCES:
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patent: 3683550 (1972-08-01), Corlett et al.
patent: 3816960 (1974-06-01), Gudin et al.
patent: 4003156 (1977-01-01), Sibi et al.
The Control of Growth in Plant Cells, Steward, Scientific Amer., Oct. 1963, pp. 104-113.
Propagation of Asparagus --, Hasegawa et al., J. Amer. Soc. Hort. Sci., 98(2), pp. 143-148, 1973.
Propagation of --, Meyer et al., Hortscience, Oct. 1975, pp. 479-480.

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