DNA fragment encoding a polypeptide having nitrile hydratase act

Details DNA fragment encoding a polypeptide having nitrile hydratase act DNA fragment encoding a polypeptide having nitrile hydratase act

1993-03-09

1998-03-24

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing nitrogen-containing organic compound

435227, 4353201, 4352523, 435325, 43525411, 4351723, 536 23, C12P 1302, C12N 100, C12N 500, C12N 978, C12N 1555, C12N 1563

Patent

active

057311765

ABSTRACT:
The present invention has disclosed the amino acid sequence and nucleotide sequence of the .alpha.- and .beta.-subunits of two types of nitrile hydratase derived from Rhodococcus rhodochrous J-1. The DNA fragment encoding nitrile hydratase is inserted into an expression vector and the recombinant vector is used for transformation. The transformant contains multiple copies of the gene and can produce much higher level of nitrile hydratase compared with conventionally used microorganisms.

REFERENCES:
Nagasawa et al., "Nitrile Hydratase-Catalyzed Production of Nicotinamide from 3-Cyanopyridine in Rhodococcus rhodocrous J1" Appl. Env. Microbiol. (1988) 54(7):1766-1769.
Nishiyama et al., "Cloning and Characterization of genes Responsible for the Metabolism of Nitrile Compounds from Pseudomonas chlororaphis B23" J. Bacteriol., (1991) 173(8):2465-2472.

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