Process for the production of sarcosine oxidase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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435 17, 435832, C12D 1310

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active

042162920

ABSTRACT:
An enzyme sarcosine oxidase is produced by culturing a microorganism belonging to the genus Bacillus, and particularly the species Bacillus sp. B-0618, FERM-P No. 4049, NRRL No. B-11380, and isolating the sarcosine oxidase thus produced from the culture medium. Sarcosine oxidase is useful for the determination of creatinine in the presence of creatinase and creatininase in a sample by mixing the enzymes with the sample and then measuring the generated hydrogen peroxide, formaldehyde or glycine.

REFERENCES:
Enzyme Handbook (Japan) 1966, pp. 125-126.

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