Method of gradient separation

Details Method of gradient separation Method of gradient separation

1975-07-16

1977-04-05

Krizmanich, George H.

Chemistry: physical processes

210DIG23, B04B 502

Patent

active

040157750

ABSTRACT:
Zonal separations by centrifugation are achieved by first preparing a fluid density gradient in stationary, vertically disposed containers each having a length which exceeds its diameter. A sample to be separated is placed on the top of each gradient within the container. These containers are centrifuged, while maintaining their vertical orientation about a vertically oriented spin axis to reorient the fluid density gradient from vertical to horizontal and to create a horizontal separation gradient of the sample within each tube. Following centrifugation a vertical gradient is again established in each container. This vertical gradient facilitates withdrawal of the now separated particles from the several separation zones. The relatively short path length of the horizontal gradient provides a relatively steep horizontal gradient which during centrifugation speeds up the separation process. The relatively steep horizontal gradient also aids in establishing sharp, narrow separation bands, while the reorientation back to vertical facilitates their examination.

REFERENCES:
patent: 3243105 (1966-03-01), Anderson
patent: 3459369 (1969-08-01), Marks
patent: 3768727 (1973-10-01), Proni
patent: 3850369 (1974-11-01), Bull et al.
Beckman/Spinco Publication SBE-3, Analytical Ultracentrifuge, Model E, May 1960.
National Cancer Institute Monograph, Zonal Centrifuge, N. G. Anderson et al. No. 21, pp. 253-283, June, 1976.
Beckman Instruments Catalog, Analytical Cells, Spinco SB-189E, Dec. 1973, p. 11.
Analytical Biochemistry Publication, "Density Gradient Centrifugatin in Angle Head Rotors" pp. 477-478, Fisher et al. 1964.

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