Sterilizable gas permeable container for use in culturing living

Chemistry: molecular biology and microbiology – Apparatus – Bioreactor

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210501, 215261, 215305, 422101, 422102, 435297, 435298, 436810, C12M 122, C12M 124, B01L 300

Patent

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049353713

ABSTRACT:
A container for use in culturing living cells includes an enclosable portion, or base, and a cover for enclosing the enclosable portion, a path defined between the junction of the enclosable portion and the cover and a gas permeable filter positioned within the path. Gases flow into and out of the container through the filter. Vapor phase hydrogen peroxide is degraded into water vapor and oxygen by a material associated with the filter to prevent hydrogen peroxide from entering the container.

REFERENCES:
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patent: 4368081 (1983-01-01), Hata et al.
patent: 4410630 (1983-10-01), Zierdt
patent: 4521375 (1985-06-01), Houlsby
Holst, "Hydrogen Peroxide as an Oxygen Source for Immobilized Glucanobacter Oxydans," Applied Microbiology and Biotechnology, vol. 22, pp. 383-388 (1985).
Jones et al. "Influence on Added Catalase on Chromosome Stability and Neoplastic Transformation of Mouse Cells in Culture," British Journal of Cancer, vol. 52, pp. 583-590 (1985).

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