Preparation of strains of Bacillus thuringiensis having an impro

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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43525231, 435320, 4351723, 935 72, 935 11, 71 6, C12N 120, C12N 100, C05F 1108

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049353535

ABSTRACT:
The invention relates to a strain of Bacillus thuringiensis, GC 91, a sample of which has been deposited under the accession number NCTC 11821, or a derivative or mutant thereof having entomocidal activity against lepidopterous pests. The invention also relates to a process for producing a strain of Bacillus thuringiensis having improved entomocidal properties by combining into a single strain by plasmid transfer the different entomocidal properties of two respective starting strains. The new strains thus produced are useful in entomocidal compositions.

REFERENCES:
Mating Between Bacillus subtilis and Bacillus thuringiensis and transfer of cloned crystal genes, Klier et al, Mol. Gen Genet. (1983) 191:257-262.
Genetics in Relation to Insect Management, Dulmage, H. T. (1979).
J. Appl. Bacteriol. 1985, 58 (5), 437-48.
Chemical Abstracts, vol. 95, No. 7, 17 Aug. 1981, p. 343, Abstract No. 57938C.
Plasmid 1981, 5 (3), 351-65.
Arch. Microb. (1982) 132(2) pp. 159-162.
Microb. Contr. Pest. Plant Disease 1970-80 (1981) pp. 299-310-also references 2, 3 and 5 cited in EPO.
Mol. Gen. Genet. (1983) 191, pp. 257-262.
Howard T. Dulmage, et al, Journal of Invertebrate Pathology, vol. 15, No. 1, Jan. 1970, Coprecipitation With Lactose as a Means of Recovering . . . .
Howard T. Dulmage, et al, U.S. Department of Agriculture, ARM-S-30/Oct. 1982, Bacillus Thuringiensis Cultures Available from the U.S. Department . . . .
Collection De Souches De Bacillus thuringiensis, 1983, Du, Centre Collaborateur O.M.S. De Recherches et De References Pour les Bacteries Sporulees . . . .
P. Jarrett, FEMS Microbiology Letters 16 (1983), 55-60, Comparison of Plasmids From Twelve Isolates of Bacillus thuringiensis H-Serotype 7.
P. Jarrett, Journal of Applied Bacteriology, 1985, 58, 437-448, Potency Factors in the Delta-Endotoxin of Bacillus thuringiensis Var Aizawa and . . . .
H. D. Burges, Bioassay of Bacillus thuringiensis, pp. 244-254, Techniques for the Bioassay of Bacillus thuringiensis with Galleria mellonella.
Christopher C. Payne, Journal of Invertebrate Pathology 38, 71-77 (1981), The Susceptibility of the Pea Moth, Cydia nigricana, to Infection by the . . . Rearing Pieris Brassicae L. Larvae on a Semi-Synthetic Diet, No. 4999, Aug. 21, 1965, NAT.

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