Methods and compositions for screening for anti-AIDS drugs

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 6, 4352521, 435 912, 435471, 424 92, 4243201, C12Q 170

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active

061300365

ABSTRACT:
Methods and compositions related to AIDS are disclosed. Using the methods of the present invention, candidate compounds may be screened for the ability to inhibit reverse transcriptase of human immunodeficiency virus ("HIV RT"). Active HIV RT mutants may be detected by the disclosed methods. The present invention also discloses methods for screening for compounds that inhibit HIV RT obtained from an individual patient. In another aspect, methods for testing the biological effectiveness of candidate compounds for the inhibition of HIV RT in vivo are disclosed.

REFERENCES:
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Kellam, P., et al, Fifth mutation in human immunodeficiency virus type 1 reverse transcriptase contributes to the development of high-level resistance to zidovudine, Procs of the National Academy of Sciences of the USA, Mar. 1, 1992, 89(5) pp.1934-8.
Tsai, Che-chung, et al, In vitro screening for antiretroviral agents against simian immunodeficiency virus (SIV), Antiviral Research, 14 (Aug. 1990), pp.87-98.
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Inouye et al., "Screening For Reverse Transcriptase Inhibitors And Anti-HIV Substances," Journal Of Pharmaceutical Sciences 76(11): p. S190, Abstract No. J 07-X-01, 1987.

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