Modified signal peptides

Details Modified signal peptides Modified signal peptides

1983-03-09

1987-12-08

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Miscellaneous

435253, 4351723, 435 68, 935 47, 935 48, 935 50, 935 51, C12N 1500, C12N 120, C12N 100, C12P 2100

Patent

active

047118440

ABSTRACT:
The invention discloses a method for producing modified signal peptides sequences derived from wild-type signal peptide sequences of the type that are capable of forming membrane-bound lipoproteins. Modified signal peptide sequences produced by the method of the invention are useful for increasing the secretion of heterologous gene products produced by transformed host organisms. The invention further discloses a method for producing recombinant DNA sequences in vivo.

REFERENCES:
patent: 4338397 (1982-07-01), Gilbert et al.
Lai et al, Proc. Natl. Acad. Sci., vol. 78(6), pp. 3506-3510, Jun. 1981, "Bacillus licheniformis Penicillinase Synthesized in Escherichia coli Contains Covalently Linked Fatty Acid and Glyceride".
Gray et al, J. Bact., V. 145(1), Jan. 1981, pp. 422-428, "Molecular Cloning and Expression of Bacillus licheniformis .beta.-lactamase Gene in Escherichia coli and Bacillus subtilis".
Zoller et al, Nucleic Acid Res., vol. 10, 1982, pp. 6887-6900, "Organonucleonase-Directed Mutagenesis Using M13-derived Vectorian Efficient and General Procedure for the Production of Point Mutations in any fragments of DNA.
Zweibel et al, J. Bact. V. 145(1), Jan. 1981, pp. 654-656, "Preferential Selection of Deletion Mutants of the Outer Membrane Lipoprotein Gene of Escherichia coli by Globomycin".
Vlasuk et al, J. Biol. Chem., vol. 258(11), pp. 7141-7148, Jun. 1983, "Effects of the Complete Removal of Basic Amino Acid Residues from the Sound Peptide on Secretion of Lipoprotein in Escherichia coli".
Mezes et al, J. Biol. Chem., vol. 258(18), Sep. 28, 1983, pp. 11211-11218, "Construction of a penP.DELTA.1 Bacillus licheniformis 7441C .beta.-Lactamase Lacking Site for Lipoprotein Modification".
Lin et al, J. Bact., Feb. 1980, pp. 550-557, vol. 151(2), "Assembly of Outer Membrane Lipoprotein in an Escherichia coli Mutants with a Single Amino Acid Replacement within the Signal Sequence of Prolipoprotein".
Neugebauer et al, Nucl. Acid Res., vol. 9(11), 1981, pp. 2577-2588, "Penicillinase from Bacillus licheniformis: Nucleotide Sequence of the Gene and Implications for the Biosynthesis of a Secretory Protein in a Gram-Positive Bacterium".
Nielsen et al, J. Biol. Chem., vol. 257(8), Apr. 25, 1982, pp. 4490-4495, "Membrane-Bound Penicillinases in Gram-Positive Bacteria".
Inouye et al., The EMBO Journal, vol. 2, No. 1, pp. 87-91, 1983.

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