Universal eubacteria nucleic acid probes and assay methods

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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935 77, 935 78, C12Q 168

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active

054016312

ABSTRACT:
Nucleic acid probes capable of hybridizing to rRNA of eubacteria and not to rRNA of non-eubacteria are described along with methods utilizing such probes for the detection of eubacteria in clinical and other samples. Preferred embodiments include probes capable of distinguishing between gram-positive and gram-negative bacteria.

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Carl R. Woese, "Bacterial Evolution," Micro. Biol. Reviews, 51:221-271 (1987).
David E. Kohne, "Isolation and Characterization of Bacterial Ribosomal RNA Cistrons," BioPhysical Journal 8: 1104-1118 (1968).
B. Pace and L. Leon Campbell, "Homology of Ribosomal Ribonucleic Acid of Diverse Bacterial Species with Escherichia Coli and Bacillus Stearothermophilus," Journal of Bacteriology 107: 543-547 (1971).
Delond et al., "Phylogenetic Stains: Ribosomal RNA-Based Probes for the Identification of Single Cells," Science, 243: 1360 (1989).
Chen et al., "Broad Range DNA Probes for Detecting and Amplifying Eubacterial Nucleic Acids," FEMS Microbiology letter, 48: 19-24 (1989).
Giovannoni S. J. et al., "Phylogenetic Group-Specific Oligodeoxynucleotide Probes for Identification of Single Microbial Cells," Journal of Bacteriology, 170: 720-726 (1988).
Carl R. Woese, "Archaebacteria," Scientific American 244, Jun. Issue #6, 98-102 (1981).

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