Method of determining energy transfer efficiency of a donor-acce

Details Method of determining energy transfer efficiency of a donor-acce Method of determining energy transfer efficiency of a donor-acce

1991-04-10

1993-08-17

Cosimano, Edward R.

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364497, G06F 1520

Patent

active

052375153

ABSTRACT:
The energy transfer efficiency of a donor-acceptor pair of fluorescent dyes can be determined by first measuring the fluorescence and absorption spectra of donor-protein, acceptor-protein, mixture of donor-protein and acceptor-protein, and donor-acceptor-protein conjugates, then separating the respective spectra into their respective donor-protein complex and acceptor-protein complex components using multiple linear regression, and then determining the transfer efficiency on the basis of the quenching of the donor fluorescence based on the spectral data thus obtained.

REFERENCES:
patent: 4666862 (1987-05-01), Chan
patent: 4996143 (1991-02-01), Heller et al.
patent: 5051249 (1991-09-01), Metcoff
Gennis et al; "Use of Nonspecific Dye Labeling for Singlet Energy-transfer Measurements in Complex Systems. A Simple Model": Biochemistry, vol. 11 No. 13, 1972, pp. 2509-2517.
Neter et al: "Applied Linear Regression Models"; Irwin Inc.; ISBN 0-256-070768-7; 1989 pp. xiii, 225-236.
Beebe et al: "An Introduction to Multivariate Calibration and Analysis"; Analytical Chemistry, vol. 59 No, 17, Sep. 1, 1987; pp. 1007A-1017A.
Gemperline et al: "Background Correction in Multicomponent Spectroscopic Analysis Using Target Transformation Factor Analysis"; Applied Spectroscopy; vol. 41 No. 3; 1987 pp. 454-459.

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