Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-01-28
1998-07-28
Grimes, Eric
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
4351721, 4351723, 4352523, 43525233, 43525411, 43525421, 4353201, 514 29, 536 72, 536 231, C12P 1962
Patent
active
057861811
ABSTRACT:
A process for producing high purity 6,12-dideoxyerythromycin A using recombinant DNA technology is disclosed. The erythromycin producing strain, Saccharopolyspora erythraea, lacking the erythromycin C-12 and C-6 hydroxylases produces a mixture of 6,12-dideoxyerythromycin A and the precursor molecule, 6-deoxyerythromycin D. To achieve conversion of the precursor to the final product, a second copy of eryG is inserted into a non-essential region of the Sac. erythraea chromosome resulting in high purity 6,12-dideoxyerythromycin A.
REFERENCES:
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Maine Gregory T.
Post David A.
Satter Mark T.
Stassi Diane L.
Abbott Laboratories
Casuto Dianne
Danckers Andreas M.
Grimes Eric
Nashed Nashaat T.
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