Methods, acridan compounds and kits for producing light

Details Methods, acridan compounds and kits for producing light Methods, acridan compounds and kits for producing light

1996-05-09

1998-03-03

Green, Lora M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 79, 435 791, 435 792, 435 793, 435 794, 435 795, 435 18, 435 28, 435966, 435968, 435975, G01N 33535

Patent

active

057232954

ABSTRACT:
A chemiluminescent assay method, compositions, kits and chemiluminescent acridan compounds are described which use a two-step chemiluminescent reaction process. The reaction involves an acridan compound, preferably a derivative of an N-alkylacridan-9-carboxylic acid, which undergoes a reaction with a peroxide compound, a peroxidase enzyme and an enhancer under conditions of time, temperature and pH which permit the accumulation of an intermediate compound, which is subsequently induced to produce a burst of light by raising the pH. The result is generation of very high intensity light from the reaction. The peroxidase enzyme is present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the hydrolytic enzyme is bound to a reporter molecule. The method is particularly amenable to automated assays because of the separation of the incubation and light generating steps.

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