Method of fluorescent detection of nucleic acids and cytoskeleto

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 435808, 435810, 436501, 436 63, 436164, 436172, 536 221, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 536 266, 935 77, 935 78, C12Q 168

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057232881

ABSTRACT:
Disclosed is a method of fluorescent detection of a nucleic acid. The method comprises contacting to the nucleic acid a bis-dicationic aryl furan compound, such as acceptable salts thereof, and exposing the nucleic acid to light at a frequency to induce fluorescence of the compound. A method for fluorescent detection of cytoskeleton elements, and novel bis-dicationic aryl furan compounds are also disclosed.

REFERENCES:
A. Redi et al; Pericentromeric heterochromatin and A-T contents during Robertsonian fusion in the house mouse, Chromosome 91: 31-35 (1986).
D.E. Rooney et al; Human Cytogenetics: A Practical Approach, IRL Press at Oxford University Press, pp. 6-15, 22-23, 36-43, 91-119, 128-129, 162-199, 206-211 (1992).
E. A. Steck et al., "Leishmania donovani, Plasmodium berghei, Trypanosoma rhodesiense: antiprotozoal effects of some amidine types", Chemical Abstracts, vol. 96, No. 17, 26 Apr. 1982, Abstract No. 135352, p. 29, column 2.
Dykstra, C., et al, Synthesis and Characterization of a Novel Series of Aromatic Dicationic Furans With DNA-Specific Fluorescence Properties, pp. 1-7, (1994).
Wilson, W., et al, The Search for Structure-Specific Nucleic Acid-Interactive Drugs: Effects of Compound Structure on RNA versus DNA Interaction Strength, Biochemistry, 32:4098 (1993).
Bottiroli, et al., DNA Double Staining for a Fluorescence Energy Transfer Study of Chromatin in Liver Cells, Cell Biophysics, 15:249 (1989).
Grossgebauer, A New Fluorescence Technique for Staining of Mononuclear Phagocytes, Blut, 39:281-283 (1979).
Rayburn, et al., Short Note Estimating Percentage Consitutive Heterochomatin by Flow Cytometry, Experimental Cell Research, 198:175-178 (1992).
Babu, A., et al., Expression of Heterochromatin by Restriction Endonuclease Treatment and Distamycin A/DAPI Staining of Indian Muntjac (Muntiacus Muntjak) Chromosomes, Cytogenet Cell Genet 41:96 (1986).
Parolin, C., et al., The Effect of the Minor Groove Binding Agent DAPI (2-amidino-diphenyl-indole) on DNA-Directed Enzymes: an Attempt to Explain Inhibition of Plasmid Expression in Escherichia coli, FEMS Microbiology Letters 68:341 (1990).
Buys, C.H.C.M., et al., A Comparison of the Effect of 5-Bromodeoxyuridine Substitution on 33258 Hoechst-and DAPI-Fluorescence of Isolated Chromosomes by Bivariate Flow Karyotyping, Histochemistry 84:462 (1986).
Mohan, S., et al., Flexibility of DNA In 2:1 Drug-DNA Complexes-Simultaneous Binding of Two DAPI Molecules to DNA, J. Biomolecular Structure and Dynamics 9:695 (1991).
Kapuscinski, J., Interactions of Nucleic Acids with Fluorescent Dyes: Spectral Properties of Condensed Complexes, J. Histochemistry and Cytochemistry 38:1323 (1990).
Jansen, K., et al., Binding of DAPI Analogue 2,5-Bis(4-Amidinophenyl)furan to DNA, Biochemistry 32:6605 (1992).
Bonaly, J., et al., A Flow Cytometric Study of DNA Staining In Situ in Exponentially Growing and Stationary Euglena gracilis, Cytometry 8:42 (1987).
Heng, H.H.Q., et al., Modes of DAPI Banding and Simultaneous in situ Hybridization, Chromo. 326-332 (1993).
Montag, M., et al., Working with the Confocal Scanning UV-Laser Microscope: Specific DNA Localization at High Sensitivity and Multiple-Parameter Fluorescence, J. Microscopy 163:201 (1991).
Bonne, D., et al., 4',6-Diamidino-2-phenylindole, A Fluorescent Probe for Tubulin and Microtubules, J. Biological Chemistry 260:2819 (1985).
Das, B.P., et al., 1,4-Bis(4-guanylphenylethyl)benzenes as Potential Antitrypanosomal Agents, J. Pharmaceutical Sciences 71: 465 (1982).
Das, B.P., et al., Synthesis and Antiprotozoal Activity of 2,5-Bis(4-guanylphenyl)furans, J. Medicinal Chemistry 20:531 (1977).
Das, B.P., et al., Synthesis and Antitrypanosomal Activity of Some Bis(4-guanylphenyl) Five-and Six-Membered Ring Heterocycles, J. Medicinal Chemistry 23:578 (1980).
Das, B.P., et al., Synthesis and Antiprotozoal Activity of 2,5-Bis(4-guanylphenyl)thiophenes and -pyrroles, J. Medicinal Chemistry 20:1219 (1977).
Yamamoto, K., et al., DNA Recoginition and Novel Site-Specific Alkylation by Duocarmycin A with Distamycin A, Biochemistry 32:1059 (1993).
Miyakawa, I., et al., Isolation of Morphologically Intact Mitochondrial Nucleoids from the Yeast, Saccharomyces cerevisiae, J. Cell Science 88:431 (1987).
Myc, A., et al., DNA Stainability in Aneuploid Breast Tumors: Comparison of Four DNA Fluorochromes Differing in Binding Properties, Cytometry 13:389 (1992).
Dam, O., et al., Trypanocide Diamidine mit drei isolierten Ringsystemen, Anal. Chem. 11:160 (1975).
Wilson, W.D., et al., The Effects of Ligand Structure on Binding Mode and Specificity in the Interaction of Unfused Aromatic Cations with DNA. Molecular Basis of Specificity in Nucleic Acid-Drug Interactions, pp. 331-353 (1990).
Wilson, W.D., et al., Molecular Factors that Control the Nucleic Acid Binding Mode Selection by Unfused Aromatic Cations. Structure and Function: Nucleic Acids 1:83 (1992).

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