Secondary alcohol dehydrogenase enzyme and use thereof

Details Secondary alcohol dehydrogenase enzyme and use thereof Secondary alcohol dehydrogenase enzyme and use thereof

1979-03-27

1980-12-23

Penland, R. B.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435150, 435157, 435160, 435250, 435253, 435822, 435858, C12P 726, C12P 704, C12N 130, C12N 120

Patent

active

042411840

ABSTRACT:
Disclosed is a newly-discovered and isolated enzyme which is a nicotinamide adenine dinucleotide (NAD.sup.+)-linked C.sub.3 -C.sub.6 secondary alcohol-specific dehydrogenase. This enzyme, in the presence of oxygen and NAD, is capable of converting C.sub.3 -C.sub.6 secondary alcohols to the corresponding methyl ketones. The purified enzyme has a molecular weight of 95,000 .+-. 3,000 dalton as determined by acrylamide gel electrophoresis and contains two zinc atoms per molecule of protein. Primary alcohols are not oxidized by this enzyme.

REFERENCES:
patent: 3326771 (1967-06-01), Leavitt
patent: 3344037 (1967-09-01), Leavitt
patent: 3930947 (1976-01-01), Morinaga
Colby et al., "The Soluble Methane Mono-oxygenese of Methylococcus capsulatus", Biochem. J., vol. 165, No. 2 (1977), pp. 395-402.
Patel et al., "Microbial Oxidation of CH.sub.4 & CH.sub.3 OH, Crystallization & Properties of Methanol Dehydrogenase from Methylosinus sporium", J. Bact., vol. 128, No. 1 (1976), pp. 413-424.
Thomson et al., "Acetone Production by Methylobacteria", Chemical Abstracts, vol. 85, p. 240 (1976), Abstract No. 156225t.
Leadbetter et al., "Methane Utilizing Bacteria", Chemical Abstracts, vol. 54 (1958), Abstract No. 4747c.

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