Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1991-05-24
1993-08-17
Benzion, Gary
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352404, 43524048, 43524049, 43524054, 800200, 800DIG49, 800DIG50, 800DIG51, 47 58, A01H 400, A01H 700, C12N 504
Patent
active
052368416
ABSTRACT:
The invention is a method for reproducing coniferous trees by somatic embryo-genesis using plant tissue culture techniques in a multistage culturing process. A suitable explant, typically the fertilized embryo excised from an immature seed, is first cultured on a medium that induces multiple early stage proembryos. These are multiplied in a second culture having reduced growth hormones. The early stage embryos may then be placed in or on a late stage proembryo development culture with a significantly higher osmotic potential than the previous stage or stages in order to develop very robust late stage proembryos having at least 100 cells. Culturing from this point continues in a cotyledonary embryo development medium having a raised osmotic potential. Exogenous abscisic acid is now required at this stage. The concentration of abscisic acid is decreased over time to low levels either by the use of an adsorbent such as activated charcoal or by stepwise subcultures in which the later cultures have lower ABA concentration. After a period of several weeks somatic embryos having the appearance of zygotic embryos will have formed. These may be germinated before or after storage and transplanted to soil for further growth.
REFERENCES:
patent: 4217730 (1980-08-01), El-Nil
patent: 4957866 (1990-09-01), Gupta et al.
patent: 5034326 (1991-07-01), Pullman et al.
patent: 5036007 (1991-07-01), Gupta et al.
patent: 5041382 (1991-08-01), Gupta et al.
Gupta et al. (1987) Biotechnology vol. 5, Jul. pp.-710-712.
Becwar, M. R. and R. P. Feirer 1989 Factors regulating loblolly pine (Pinus taeda L.) somatic embryo development. Institute of Paper Chemistry Report, Southern Forest Tree Improvement Conference, Raleigh, N.C. Jun. 1989 pp. 178-185.
Becwar, M. R., R. Nagmani, and S. R. Wann 1990 Initiation of embryogenic cultures and somatic embryo development in lobloly pine (Pinus taeda). Canadian Journal of Forest Research 20: 810-817.
Becwar, M. R., T. L. Noland, and S. R. Wann 1987 A method for quantification of the level of somatic embryogenesis among Norway spruce callus lines. Plant Cell Reports 6:35-38.
Becwar, M. R., S. R. Wann, and R. Nagmani 1988 A survey of initiation frequency of embryogenic callus among ten families of Pinus taeda (loblolly pine). Abstracts, 4th International Conifer Tissue Culture Work Group, Aug. 8-12, 1988, Saskatoon, Saskatchewan, Canada.
Boulay, M. P., P. K. Gupta, P. Krogstrup, and D. J. Durzan 1988 Development of somatic embryos from cell suspension cultures of Norway spruce (Picea abies Karst.). Plant Cell Reports 7:134-137.
Bourgkard, F. and J. M. Favre 1988 Somatic embryos from callus of Sequoia sempervirens. Plant Cell Reports 7:445-448.
Buchheim, Julie A., Susan M. Colburn, and Jerome P. Ranch 1989 Maturation of soybean somatic embryos and the transition to plantlet growth. Plant Physiology 89:768-775.
Durzan, D. J. and P. K. Gupta 1987 Somatic embryogenesis and polyembryogenesis in Douglas-fir cell suspension cultures. Plant Science 52: 229-235.
Finer, John J., Howard B. Kriebel, and Michael R. Becwar 1989 Initiation of embryogenic callus and suspension cultures of eastern white pine (Pinus strobus L.). Plant Cell Reports 8:203-206.
Gupta, Pramod K. and Don J. Durzan 1985 Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana). Plant Cell Reports 4:177-179.
Gupta, Pramod K. and Don J. Durzan (cont.) 1986a Somatic polyembryogenesis from callus of mature sugar pine embryos. Bio/Technology 4:643-645.
1986b Plantlet regeneration via somatic embryogenesis from subcultured callus of mature embryos of Picea abies (Norway spruce). In Vitro Cellular and Developmental Biology 22:685-688.
1987 Biotechnology of somatic polyembryogenesis and plantlet regeneration in loblolly pine. Bio/Technology 5:147-151.
Hakman, Inger and Saravon Arnold 1985 Plantlet regeneration through somatic embryo-genesis in Picea abies (Norway spruce). Journal of Plant Physiology 121:149-158.
1988 Somatic embryogenesis and plant regeneration from suspension cultures of Picea glauca (white spruce). Physiologia Plantarum 72:579-587.
Hakman, Inger, L. C. Fowke, S. von Arnold, and T. Eriksson 1985 The development of somatic embryos in tissue cultures initiated from immature embryos of Picea abies (Norway spruce). Plant Science 38:33-35.
Johansson, Lars 1983 Effects of activated charcoal in another cultures. Physiologia Plantarum 59:397-403.
Johansson, Lars, Barbro Andersson, and Tage Eriksson 1982 Improvement of anther culture technique: activated charcoal bound in agar medium in combination with liquid medium and elevated CO.sub.2 concentration. Physiologia Plantarum 54:24-30.
Lu, Chen-Yi, and Trevor A. Thorpe 1987 Somatic embryogenesis and plantlet regeneration in cultured immature embryos of Picea glauca. Journal of Plant Physiology 128:297-302.
Murashige, Toshio and Folke Skoog 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473-493.
Nagmani, R. and J. M. Bonga. 1985 Embryogenesis in subcultured callus of Larix decidua. Canadian Journal of Forest Research 15:1088-1091.
Nagmani, R. and M. R. Becwar 1988 Factors affecting somatic embryo development in loblolly pine. Abstracts, 4th International Conifer Tissue Culture Work Group, Aug. 8-12, 1988, Saskatoon Saskatchewan, Canada.
Raghavan, V. N. 1986 Experimental Embryogenesis, p. 100, McMillan, New York.
Schuller, Astrid and Gerhard Reuther 1989 Response of Abies alba embryonal-suspensor mass to various carbohydrate treatments. Somatic Cell Genetics Working Party S2-04-07 and NATO Advanced Research Workshop on Woody Plant Biology, Institute of Forest Genetics, Placerville, California, Oct. 15-19, 1989 (Abstract).
Singh, Hardev 1978 "Embryo" in Embryology of Gymnosperms, Chapter 11, Gebruder Borntrager, Berlin.
Teasdale, Robert D., Pamela A. Dawson, and H. W. Woolhouse. 1986 Mineral nutrient requirements of a loblolly pine (Pinus taeda) cell suspension culture. Plant Physiology 82:942-945.
Verhagen, S. and S. R. Wann 1989 Norway spruce somatic embryogenesis: high frequency initiation from light colored mature embryos. Plant Cell Tissue and Organ Culture 16:103-111.
Von Arnold, Sara 1987 Improved efficiency of somatic embryogenesis in mature embryos of Picea abies (L.) Karst. Journal of Plant Physiology 128:233-244.
Von Arnold, Sara and Inger Hakman 1988 Regulation of somatic embryo development in Picea abies by abscisic acid (ABA). Journal of Plant Physiology 132:164-169.
Yeung, Edward C. and D. C. W. Brown 1982 The osmotic environment of developing embryos of Phaseolus vulgaris. Z. Pfanzenphysiol. Bd. 106 S.:149-156.
Ziv, Meira and Geula Gadasi 1986 Enhanced embryogenesis and plant regeneration from cucumber (Cucumis sativus L.) callus by activated charcoal in solid/liquid double-layer cultures. Plant Science 47:115-122.
Gupta Pramod K.
Pullman Gerald S.
LandOfFree
Method for reproducing conifers by somatic embryogenesis using s does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for reproducing conifers by somatic embryogenesis using s, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for reproducing conifers by somatic embryogenesis using s will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2243318