Device for enhancing fluorescence and kinetics and methods of us

Details Device for enhancing fluorescence and kinetics and methods of us Device for enhancing fluorescence and kinetics and methods of us

1992-02-12

1993-08-17

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 29, 435968, 436800, C12Q 104

Patent

active

052368270

ABSTRACT:
A carrier having at least one kinetics and fluorescence enhancing support and a dry substance selected from the group consisting of fluorogenic substrates, B methylumbelliferone, 7-amino-4-methyl coumarin, B-napthylamine, fluoroscein, and resorufin deposited on the support demonstrates substantial enhancement of hydrolysis kinetics and fluorescence over pure liquid systems. When the device has a plurality of supports and the supports have different fluorogenic substrates an enzyme rate-of-reaction profile representative of a microorganism in the suspension can be determined and used to identify the organism. The device can also be used to characterize enzymes expressed by other biological specimens.

REFERENCES:
patent: 4780423 (1988-10-01), Bluestein et al.
patent: 4868106 (1989-09-01), Ito et al.
patent: 4919890 (1990-04-01), Arai et al.
patent: 4959305 (1990-09-01), Woodrum
patent: 4965087 (1990-10-01), Wolfbeis

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