Production of proteins in procaryotes

Details Production of proteins in procaryotes Production of proteins in procaryotes

1985-02-22

1989-08-29

Phillips, Delbert R.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Hormones, e.g., prolactin, thymosin, growth factors, etc.

C07K 1300

Patent

active

048618683

ABSTRACT:
A method for preparing polypeptides in bacteria with an alanine rather than a methionine at the N-terminus. The DNA sequence expressed has an alanine codon immediately following from one to about three contiguous methionine codons including a translation start signal and allows for the expression of polypeptides having the amino acid sequence of, for example, naturally occurring eucaryotic proteins such as various bovine and porcine somatotropin species.

REFERENCES:
patent: 3842063 (1974-10-01), Chance et al.
patent: 4056520 (1977-11-01), Sonenberg et al.
patent: 4409141 (1983-10-01), Noda et al.
patent: 4423037 (1983-12-01), Rosenblatt et al.
patent: 4443539 (1984-04-01), Fraser et al.
patent: 4521409 (1985-06-01), Bauman
patent: 4549986 (1985-10-01), Evans et al.
Methods in Enzymology 80:680-702 (1981) Goldberg et al.
Cell 20:215-222 (1980) Sherman et al.
Cell 20:543-553 (1980) Guarente et al.
J. Biological Chem. 246:7429-7445 (1971) Stewart et al.
Biological Chemistry 2nd ed. (1971) Mahler & Cordes eds. pp. 929-933, 973-978.
J. Molecular Biol. 7:483-496 (1963) Waller, J. P.
Proc. Nat'l. Acad. Sci. U.S.A. 81:5403-5407 (1984) Schoner et al.
Nucleic Acid Research 9:19-30 (1981) Keshet et al.
J. Biological Chem. 255:7521-7524 (1980) Miller et al.
Proc. Nat'l. Acad. Sci. U.S.A. 74:2432-2436 (1977) Lingappa et al.
In "Recent Progress in Hormone Research" (1973) vol. 29 pp. 387-416 Niall et al.
European J. Biochem. 37:164-170 (1973) Santome et al.
FEBS Letters 35:11-14 (1973) Wallis, M.
FEBS Letters 18:53-54 (1971) Fernandez et al.
J. Biological Chem. 245:3407-3415 (1970) Mills et al.
FEBS Letters 3:118-120 (1969) Wallis, M.
J. Biological Chem. 244:1567-1575 (1969) Fellows and Rogol.
Biochem. J. 223:1-13 (1984) Reinhart et al.
Biochem. J. 218:573-581 (1984) Hart et al.
Bio/Technology (Feb. 1985) pp. 151-154 Schoner et al.
Proc. Nat'l. Acad. Sci. U.S.A. 80:21-25 (1983) de Boer et al.
DNA 2:37-45 (1983) Seeburg et al.
In "Promoters: Structure and Function" (1982) eds. Rodriguez and Chamberlin pp. 462-481.
Nature 281:544-548 (1979) Goeddel et al.
Ann. Rev. Genetics 13:319-353 (1979) Rosenberg and Court.
J. Bacteriology 133:1457-1466 (1978) Miozzari and Yanofsky.
Biochem. Biophys. Res. Comm 56:168-176 (1974) Graf and Li.
V. M. Vogt, "Purification and Properties of an Aminopeptidase from Escherichia coli", 245 (18) J. Biol. Chem. 4760-4769 (1970).
A. T. Matheson et al., "A ribosomal-bound aminopeptidase in Escherichia coli B:substrate specificity", 48 Can. J. Biochem. 1292-1296 (1970).
M. Takeda et al., "Protein Chain Initiation and Deformylation in B. subtilis Homogenates:," 60 Proc. Natl. Acad. Sci. 1487-1494 (1970).
Fred Sherman and John W. Stewart, "Mutations Altering Initiation of Translation of Yeast Iso-1-cytochrome c; Contrasts between the Eukaryotic and Prokaryotic Initiation Process", The Molecular Biology of the Yeast Saccharomyces, Metabolism and Gene Expression, published by Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724 pp. 301-333 (1982).
Machlin, L. J., "Effect of Growth Hormone on Milk Production and Feed Utilization in Dairy Cows," J. of Dairy Sci., vol. 56, No. 5 (1973), pp. 575-580.

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