Chemistry: analytical and immunological testing – Biospecific ligand binding assay
Patent
1987-07-24
1989-08-29
Kepplinger, Esther M.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
436522, 436525, 436527, 436529, 436530, 436531, 436 66, 436 67, 436800, 436815, G01N 33566, G01N 3372
Patent
active
048617288
ABSTRACT:
A method for determining the percentage of glycosylated hemoglobin in the total hemoglobin of a blood sample includes binding of both glycosylated and nonglycosylated hemoglobin competitively to a nonspecific binder for hemoglobin affixed to a dipstick and reacting the glycosylated hemoglobin with a dihydroxyboryl reagent conjugated to a fluorescent dye. When the binding and reacting steps are complete, the dipstick is washed and its color is measured by absorption of incident light having a wavelength within the absorption range of hemoglobin as an indication of total hemoglobin in the sample. Incident light having a wavelength within the absorption range of the dye is then applied to the dipstick and fluorescence from the dipstick is measured as an indication of glycosylated hemoglobin. The measurements may be made with a reflectometer capable of computing the percentage of glycosylated hemoglobin in the sample from the color and fluorescence measurements. The invention includes a kit of materials useful for performing the assay method of the invention.
REFERENCES:
patent: 4222836 (1980-09-01), Kerr et al.
patent: 4269605 (1981-05-01), Dean et al.
patent: 4270921 (1981-06-01), Graas
patent: 4351711 (1982-09-01), Ambler
patent: 4371374 (1983-02-01), Cerami et al.
patent: 4389491 (1983-06-01), Hanamoto et al.
patent: 4407961 (1983-10-01), Sanders
patent: 4409335 (1983-10-01), Hanamoto et al.
patent: 4436820 (1984-03-01), Reiter
patent: 4658022 (1987-04-01), Knowles et al.
patent: 4777128 (1988-10-01), Lippa
Yoshitake et al., Conjugation of Glucose Oxidase from Aspergillus niger and Rabbit Antibodies Using N-Hydroxysuccinimide Ester of N-(4-Carboxycyclohexylmethyl)-Maleimide, Eur. J. Biochem. 101, 395-9 (1979).
Carlsson et al., Protein Thiolation and Reversible Protein-Protein Conjugation, Biochem. J. 173, 723-737 (1978).
Becton Dickinson and Company
Brown Richard E.
Kepplinger Esther M.
Wagner Richard
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