Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1993-11-12
1996-06-18
Martinell, James
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 231, 4352523, 4353201, C12N 1500, C12N 1512
Patent
active
055278973
ABSTRACT:
Disclosed herein are Human Id genes (Id-1H and Id-1H') and variants thereof. Origin, preparation process, biochemical functions and the like: two synthetic oligonucleotides separately having base sequences corresponding to regions of amino acid sequence, which are almost completely conserved by three mouse Id genes (Id-1, Id-2 and HLH462) are used as probes to screen clones having respective cDNAs complementary to the probes from a cDNA library of human TIG-1 fibroblasts in accordance with the hybridization method, thereby isolating human Id genes. The expression of the human Id genes specifically varies according to the state of cell proliferation, in particular, the acquisition of cell aging and permanently proliferating ability.
Expression vectors with the human Id gene or variant thereof integrated therein, transformed cell strains with the expression vector introduced therein, diagnostic probes obtained by chemically synthesizing a partial sequence of the base sequence of the human Id gene, or a variation sequence thereof, and antisense oligonucleotides composed of a base sequences complementary to the partial sequence of the base sequence of the human Id gene, or the variation sequence thereof are also disclosed.
REFERENCES:
Suggs et al, Proc. Natl. Acad. Sci. USA 78: 6613 (1981).
Marcus-Sekura, Anal. Biochem. 172: 289 (1988).
The Protein ID: A Negative Regulator of Helix-Loop-Helix DNA Binding Proteins, Robert Benezra et al., Cell, vol. 61, pp. 49-59, Apr. 1990.
An ID-Related Helix-Loop-Helix Protein Encoded by a Growth Factor-Inducible Gene, Barbara Christy et al., Proc. Natl. Acad. Sci., vol. 88, pp. 1815-1819, Mar. 1991.
A Human ID-Like Helix-Loop-Helix Protein Expressed During Early Development, Joseph Biggs et al., Proc. Natl. Acad. Sci, vol. 89. pp. 1512-1516, Feb. 1992.
B. Kreider et al., "Inhibition of myeloid differentiation by the helix-loop-helix protein Id", Science, vol. 255, 1992, pp. 1700-1702.
J. P. Springhorn et al., "Transcriptional regulation in cardiac muscle", Journal of Biological Chemistry, vol. 267, 1992, pp. 14360-14365.
W. Ellmeier et al., "Mutually exclusive expression of a helix-loop-helix gene and N-myc in human neuroblastomas and in normal development", The EMBO Journal, vol. 11, 1992, pp. 2563-2571.
Hara Eiji
Kishimoto Toshihiko
Nakada Susumu
Nakamura Takeshi
Oda Kinichiro
Martinell James
Sumitomo Electric Industries Ltd.
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