Methods for determining effects of a compound on the activity of

Details Methods for determining effects of a compound on the activity of Methods for determining effects of a compound on the activity of

1995-01-17

1998-07-28

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 32, 435184, C12Q 126, C12Q 118, C12N 999

Patent

active

057861668

ABSTRACT:
A screening method to determine whether a chemical inhibits the function of a class of bacterial periplasmic oxidoreductase enzymes, exemplified by TcpG of Vibrio cholerae, DsbA E. coli, and Por of Haemophilus influenzae, id disclosed.

REFERENCES:
"pTrc 99 A Expression Vector Kit", Pharmacia Biotech, p. 116 Amman. Gene vol. 69:301 1988.
James C. Bardwell and Jon Beckwith, "The Bonds That Tie: Catalyzed Disulfide Bond Formation", Cell, vol. 74, 769-771 (1993).
Joel A. Peek and Ronald K. Taylor, "Characterization of a periplasmic thiol:disulfide interchange protein required for the functional maturation of secreted virulence factors of Vibrio cholerae", Proc. Natl. Acad. Sci. USA, vol. 89, pp. 6210-6214 (1992).
James C. A. Bardwell, "Building bridges: disulphide bond formation in the cell", Molecular Microbiology, 14(2), 199-204 (1994).
R. E. Isaacson, P. C. Fusco, C. C. Brinton, and H. W. Moon, "In Vitro Adhesion of Escherichia coli to Porcine Small Intestinal Epithelial Cells: Pili as Adhesive Factors", Infection and Immunity, vol. 21, No. 2, pp. 392-397 (1978).
Jun Yu, Helen Webb, and Timothy R. Hirst, "A homologue of the Escherichia coli DsbA protein involved in disulphide bond formation is required for enterotoxin biogenesis in Vibrio cholerae", Molecular Microbiology, 6(14), 1949-1958 (1992).
Bardwell, J. Identification of a protein required . . . cell vol. 67 581-589 1991.
Peer, J. Characterization of a periplasmic . . . Proc. Natl. Acad. Sci. USA 89 pp. 6210-6214 1992.
Yu, J. Cloning and Active Site Mutagenesis . . . J of Biol Chem 268(6) 4326-4330 Feb. 1993.

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