Polynucleotide determination with selectable cleavage sites

Details Polynucleotide determination with selectable cleavage sites Polynucleotide determination with selectable cleavage sites

1988-09-29

1992-06-02

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 435803, 435810, 436501, 436527, 536 27, 935 23, 935 78, C12Q 168

Patent

active

051186055

ABSTRACT:
Novel methods for assaying a nucleic acid analyte are provided, which employ polynucleotides having oligonucleotide sequences substantially homologous to a sequence of interest in the analyte, where the presence or absence of hybridization at a predetermined stringency provides for the release of a label from a support. Particularly, various techniques are employed for binding a label to a support, whereupon cleavage of either a single or double strand, a label may be released from a support, where the release of the label can be detected as indicative of the presence of a particular oligonucleotide sequence in a sample. The method finds use in diagnosis of disease, genetic monitoring, and analysis of nucleic acid mixtures.

REFERENCES:
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