Human and rat prolactin and preprolactin cloned genes

Details Human and rat prolactin and preprolactin cloned genes Human and rat prolactin and preprolactin cloned genes

1984-03-23

1988-02-16

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

435 68, 435 70, 435 71, 435 91, 4351721, 4351723, 435253, 435317, 435849, 536 27, 935 13, 935 29, 935 73, C12N 100, C12N 1500, C12N 120, C12P 2100, C12P 2102, C12P 2104, C12P 1934, C12R 119, C07H 2104

Patent

active

047255498

ABSTRACT:
A DNA which comprises a deoxynucleotide sequence coding for prolactin, particularly human prolactin, is described. A transfer vector and an expression vector containing this DNA and microorganisms transformed by these vectors are also described. A method for preparing a reverse transcript (cDNA) from a messenger RNA is also disclosed herein.
The invention described herein was made in the course of, or under, a grant from the National Institutes of Health.

REFERENCES:
patent: 4342832 (1982-08-01), Goeddel et al.
Chang et al, "Phenotypic Expression in E. Coli of a DNA Sequence Coding for Mouse Dihydrofolate Reductase", Nature 275:617 (1978).
Weiss et al, "Importance of Full Size Complementary DNA in Nucleic Acid Hybridization", J. Biol. Chem. 251: 3425 (1976).
McKean et al, "Complete Amino Acid Sequence of the Precursor Region of Rat Prolactin", Biochemistry 17: 5215 (1978).
Gubbins et al, "Construction and Analysis of Recombinant DNAs Containing a Structural Gene for Rat Prolactin", Nucleic Acids Res. 6: 915 (1979).
Nilson et al, "Purification of Pre-Prolactin mRNA from Bovine Anterior Pituitary Glands", J. Biol. Chem. 254: 1516 (1979).
Nilson et al, "Construction and Characterization of a cDNA Clone Containing a Portion of the Bovine Prolactin Sequence", Nucleic Acids Res. 8: 1561 (1980).
Brennessel et al, "Isolation and Characterization of Prolactin-Copy DNA", Biochem. Biophys. Res. Comm. 87: 635 (1979).

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