Promoter probe vectors

Details Promoter probe vectors Promoter probe vectors

1983-02-22

1988-02-16

Tarcza, J. E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 4351723, 435320, 935 6, 935 41, 935 29, 935 82, C12Q 168, C12P 1934, C12N 1500, C12N 100

Patent

active

047255358

ABSTRACT:
Promoter probe vectors for determining the presence of efficient promoter regions on DNA segments, which segments permit microbiological expression of genetic information. The promoter probe vector comprises replicons active in E. coli and B. subtilis, a structural gene for B-galactosidase and at least one endonuclease restriction site suitable for insertion of promoter-containing DNA fragments.

REFERENCES:
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Bibb et al, "Gene Expression in Streptomyces: Construction and Application of Promoter-Probe Plasmid Vectors in Streptomyces Lividans", Molecular and General Genetics, 187, pp. 265-277 (1982).
West et al, "Construction and Characterization of E. Coli Promoter-Probe Plasmid Vectors", Gene, 7, pp. 271-288 (1979).
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