Optimally fluorescent oligonucleotides

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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435 6, 435 911, 435 912, 536 231, 536 243, 536 2433, 536 253, 536 2532, C12Q 168, C12P 1934, C07H 1900, C07H 2102

Patent

active

060720433

ABSTRACT:
A method for the preparation of optimally fluorescent oligonucleotides wherein fluorescent dye-conjugated nucleotide triphosphates are incorporated into a nucleic acid sequence in a defined repetitive manner which allows for the optimal specific fluorescence of the oligonucleotide. The oligonucleotides of the present invention are useful in the assay of a wide variety of nucleic acid sequences, specifically wherever highly fluorescent nucleic acid probes are desired.

REFERENCES:
patent: 5403708 (1995-04-01), Brennan et al.
patent: 5652099 (1997-07-01), Conrad
New England Biolabs Catalog, (New England Biolab Inc., Beverly, MA), p. 97, 1993.

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