Novel bacteria containing a plasmid having a tRNA code

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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4351723, 435317, 935 29, 935 72, 935 73, 935 5, C12N 120, C12N 1500, C12N 100

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046543074

ABSTRACT:
A bacterium which comprises chromosomal DNA which lacks a DNA sequence to code for a tRNA for an amino acid and which bacterium further comprises a plasmid which has a DNA sequence which does effectively code for said tRNA for said amino acid. The invention further includes such a bacterium wherein the DNA sequence for the tRNA for the amino acid is located in a ribosomal RNA operon preferably between a ribosomal RNA promoter and a ribosomal RNA promoter termination sequence within the plasmid.

REFERENCES:
patent: 4338400 (1982-07-01), Manis et al.
patent: 4374200 (1983-02-01), Olsen
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Nomura, M. et al, Ann. Rev. Genet. vol. 11, pp. 297-347, 1977.
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Chakrabarty, A., Genetic Engineering, CRC Press, Inc., pp. 97-102, 1978.
Boros et al, Nucleic Acids Research, vol. 6, No. 5, 1979, pp. 1817-1830.
Morgan et al, Cell, vol. 13, pp. 335-344, 1978.
Clarke et al, Cell, vol. 9, pp. 91-99, 1976.
"Molecular Cloning a Laboratory Manual", Maniatis et al, 1982, Cold Spring Harbor Labor., pp. 100-101.
"Ribosomal RNA Genes in Escherchia Coci", Morgan, The Cell Nucleus, vol. X, 1982, pp. 1-29.
"Gene-Protein Index of Escherichia Coli K-12"; Niedhardt et al, Microbiological Reviews, Jun. 1983, pp. 231-284.

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