Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1984-06-04
2000-09-05
Carlson, Karen Cochrane
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
536 2371, 4352523, 43525233, 4352525, 435419, 4353201, C12P 2106, C07H 2104, C12N 120, C12N 1500
Patent
active
061141380
ABSTRACT:
The Bacillus thuringiensis var. kurstaki HD-73 crystal protein gene was cloned into pBR322. E. coli cells harboring this recombinant plasmid produced a 130 kD protoxin that was toxic to Manduca sexta (tobacco hornworm) larvae. Plasmids having the 3'-end of the protoxin gene deleted where also constructed. E. coli cells harboring these deleted plasmids produced an active, soluble 68 kD toxin, provided that the 3'-deletion had not removed sequences encoding the 68 kD toxin. The invention provides methods to produce 68 kD toxin protein by constructing partial protoxin genes encoding the toxin followed by expression of the genes in living cells. Useful plasmids and cells are also provided.
REFERENCES:
patent: 4448885 (1984-05-01), Schnepf et al.
U.S. Ser. No. 535,354, Adang (application).
Chang 1983 Trends Biotech. v1 100-1.
Huber et al 1981 Pathogenesis of Invertebrate Microbiol Dis. 209-34.
Whitely et al 1982 Mol. Cloning & Gene Regulation in bacilli pp. 131-144.
Wong et al 1983 J Biol Chem v 258 1960-67.
Yamamoto et al 1981 Bioch Biophys Res Comm v 103 414-21.
Schnepf et al 1985 "Delineation of a Toxin-Encoding Segment of a Bacillus thuringiensis Crystal Protein Gene" v 260 6273-80.
Chestukhina et al 1982 "The Main Features of Bacillus thuringiensis of .sigma.-Endotoxin Molecular Strucutre" Arch Micro v 132 159-162.
Carlson Karen Cochrane
Mycogen Plant Science, Inc.
Srivastava Devesh
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