Method for increased expression of low molecular weight recombin

Details Method for increased expression of low molecular weight recombin Method for increased expression of low molecular weight recombin

1991-09-24

1995-01-03

Draper, Garnette D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 694, 435 691, C12N 1500, C12N 121, C12N 1517, C12N 1518

Patent

active

053786130

ABSTRACT:
The present invention provides a method for increasing expression of low molecular weight polypeptides which are produced by recombinant DNA tranformation of prokaryotic host cells. This invention allows the direct expression of these polypeptides without fusion to an otherwise expressible protein and requires only the addition of a single amino acid to the sequence of the polypeptide product of interest.

REFERENCES:
patent: 4782139 (1988-11-01), DeMarchi et al.
patent: 5158875 (1992-10-01), Miller et al.
patent: 5304473 (1994-04-01), Belagaje et al.
Looman, A. C. et al., EMBO J, 6(8):2489-92, 1987.
DiMarchi, R. D. et al., J. Cell. Biochem., Supplement 12B, p. 58, V024.
Hsiung, H. M. et al., Methods in Enzymology, 153:390-401, 1987.
Cantrell et al., Effects of second-codon on expression of the insulin-like growth factor-II-encoding gene in Escherichia coli, Gene, 98:217-23, (1991).
Buell et al., Optimizing the expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I), Nucleic Acids Research, 13:1923-38 (1985).
Wong et al., Expression of insulin-like growth factor-I in Escherichia coli, Gene, 68:193-203 (1988).
Saito et al., Production and Isolation of Recombinant Somatomedin C, J. Biochem. 101:123.sub.]134 (1987).

Affiliated with

Also associated with

Rating

Method for increased expression of low molecular weight recombin does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for increased expression of low molecular weight recombin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for increased expression of low molecular weight recombin will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2210976