Process and nutrient medium for growing microorganism

Details Process and nutrient medium for growing microorganism Process and nutrient medium for growing microorganism

1979-11-05

1981-06-23

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435253, 435850, C12N 978, C12N 120

Patent

active

042751640

ABSTRACT:
A fermentation process and improved aqueous nutrient medium is used for the production of urease-free creatinine iminohydrolase from an aerobic soil microorganism. In the process, a fresh sample of the microorganism grown on a creatinine-containing maintenance medium is transferred to a microbial growth medium to grow the microorganism, the growing microorganism is then transferred to a production medium to produce microorganism in which creatinine iminohydrolase production has been induced, and the desired enzyme is then extracted from the microorganism. An improved aqueous nutrient medium for use as the aforementioned production medium is disclosed.

REFERENCES:
patent: 3806420 (1974-04-01), Holz et al.
patent: 3907644 (1975-09-01), Mollering et al.
patent: 4039384 (1977-08-01), Suzuki et al.
patent: 4087329 (1978-05-01), Terada et al.
Smulmajster, Journal of Bacteriology, vol. 75, pp. 633-639 (1958).
Szulmajster, Biochem. Biophys. Acta, vol. 30, pp. 154-163 (1958).

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