Absorbance assay for the measurement of cornified envelopes usin

Details Absorbance assay for the measurement of cornified envelopes usin Absorbance assay for the measurement of cornified envelopes usin

1997-03-20

1998-06-09

Snay, Jeffrey

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436164, 436178, G01N 3368

Patent

active

057632820

ABSTRACT:
A rapid, practical assay for measuring the concentration of cornified envelopes based on measuring the absorbance, which utilizes the addition of bicinchoninic acid to insoluble cornified envelopes preparation, after the cornified envelopes have been separated from soluble proteins.

REFERENCES:
patent: 4839295 (1989-06-01), Smith
patent: 5506144 (1996-04-01), Sundrehagen
patent: 5654171 (1997-08-01), Suginaka et al.
Monroe et al., "Quantitation of Cross-Linked Protein: An Alternative to Counting Cornified Envelopes as an Index of Keratinocyte Differentiation", Analytical Biochemistry, 199, pp. 25-28 (1991).
Lowry et al., "Protein Measurement With The Folin Phenol Reagent", Washington University School of Medicine, St. Louis, Missouri, May 28, 1951, pp. 265-275.
Sun et al., "Differentiation of the Epidermal Keratinocyte in Cell Culture: Formation of the Cornified Envelope", Cell, vol. 9, pp. 511-521, Dec. 1975(Part 1).
Stich, "Determination of Protein Covalently Bound to Agarose Supports Using Bicinchoninic Acid", Analytical Biochemistry, 191, pp. 343-346 (1990).
Smith et al., "Measurement of Protein Using Bicinchoninic Acid", Analytical Chemistry, 150, pp. 76-85 (1985).
Hill et al., "Protein Determination Using Bicinchoninic Acid in the Presence of Sulfhydryl Reagents", Analytical Biochemistry, 170, pp. 203-208 (1988).
Pierce Chemical Company, Product Brochure Instructions, "BCA Protein Assay Reagent Kit", USA, Jun. 1996.
Pierce Chemical Company, Product Brochure Instructions, "BCA Protein Assay Reagent", USA, Jul. 1995.

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