Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-11-17
1998-06-09
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 935 77, 935 78, C12Q 168, C12P 1934, C12N 1500
Patent
active
057631751
ABSTRACT:
The invention provides a method for sequencing each polynucleotide of a population by using an oligonucleotide tag assigned to each such polynucleotide for transfering sequence information to a tag complement located on a spatially addressable array of such complements. That is, a unique tag is attached to each polynucleotide of a population which can be copied and used to shuttle sequence information to its complement at a fixed position on an array of such complements. After a tag hybridizes with its complement, a signal is generated that is indicative of the transferred sequence information. Sequences of the tagged polynucleotides are determined by repeated cycles of information transfer and signal detection at the positions of the corresponding tag complements.
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Jones W. Gary
Lynx Therapeutics, Inc.
Macevicz Stephen C.
Whisenant Ethan
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