Multichromophore fluorescent DNA intercalation complexes

Details Multichromophore fluorescent DNA intercalation complexes Multichromophore fluorescent DNA intercalation complexes

1993-12-02

1998-06-09

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

2041828, C02Q 168, G01N 2726

Patent

active

057631620

ABSTRACT:
Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.

REFERENCES:
patent: 4737454 (1988-04-01), Dattagupta et al.
Gaugain et al. Biochemistry 17: 5078-5088 (1978) "DNA bifunctional Intercalators . . . ".
Maniatz et al "Molecular Cloning" 2nd edition, 1989 p. 6.13.
Biotechniques vol. 8, pp. 36, 38 (1990) (Biosis abstract).
Markovits et al, Analytical Biochem 94:259-264 (1979).

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