Homogenous specific binding assay reagent system and labeled con

Details Homogenous specific binding assay reagent system and labeled con Homogenous specific binding assay reagent system and labeled con

1986-01-09

1988-12-13

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435174, 436537, 436544, 436546, G01N 33532, G01N 33533, G01N 33542, C12N 1116

Patent

active

047910550

ABSTRACT:
The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction is affected by reaction between the specific binding substance in the conjugate and a specific binding counterpart thereto. The presence of a ligand in a liquid medium may be determined using competitive or displacement binding or sequential saturation techniques wherein the specific binding substance in the conjugate is the ligand or a specific binding analog thereof, or using a direct binding technique wherein the specific binding substance is a specific binding partner of the ligand. The effect of the specific binding reaction on the activity of the conjugated reactant is related to the presence or amount of the ligand in the liquid medium tested.

REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3880934 (1975-04-01), Rammler
patent: 3998943 (1976-02-01), Ullman
Miles, et al., Nature, vol. 219, 1968, pp. 186-189.

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