Process for the measurement of nucleic acids

Details Process for the measurement of nucleic acids Process for the measurement of nucleic acids

1993-06-14

1996-08-06

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168

Patent

active

055432921

ABSTRACT:
A process for detecting the existence of at least one sequence of oligonucleotide in a nucleic acid sample includes at least one performance of a step of mixing the sample with
a labeled common polynucleotide, and
a polynucleotide probe comprising a sequence complementary to at least a part of the sequence of oligonucleotide and a sequence complementary to at least a part of the labeled common polynucleotide. The existence of a sequence of oligonucleotide which is an analyte existing in a nucleic acid sample can be detected by the use of a common reagent and an unattached polynucleotide having a base sequence specific for the analyte to be measured.

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patent: 4797355 (1989-01-01), Stabinsky
patent: 4868104 (1989-09-01), Kurn et al.
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Virtanen, the Lancet p. 381-383 (1983) "Novel Test For Rapid Viral Diagnosis: Detection . . . ".
Matthews et al, "Review: Analytical Strategies for the Use of DNA Probes", Analytical Biochemistry, 169, 1988, pp. 1-25.
E. M. Southern, J. Mol. Biol., 98, 503-517, (1975).
J. Meinkoth, Anal. Biochem., 138, 267-284, (1984).

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