DNA detector and DNA detection method

Details DNA detector and DNA detection method DNA detector and DNA detection method

1994-11-07

1996-06-25

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204452, 356344, G01N 27447, G01N 2726

Patent

active

055296793

ABSTRACT:
An electrophoresis apparatus includes a plurality of first capillaries containing a separation medium, such as a gel, and an optical cell in which one end of each of the first capillaries is disposed. Samples labeled with fluorophores are introduced into the first capillaries, and an electric field is applied to the first capillaries to cause the samples to migrate through the first capillaries into the optical cell. A light source excites the fluorophores with light when the samples are in the optical cell, causing the fluorophores to emit fluorescence, and a photodetecting system detects the fluorescence emitted by the fluorophores. The ends of the first capillaries in the optical cell may be arranged in a straight line. The light source may include a He-Ne laser emitting light having a wavelength of 594 nm, and the fluorophores may include either sulforhodamine or a derivative of sulforhodamine. The apparatus may include a plurality of second capillaries each having one end disposed in the optical cell such that the ends of the second capillaries are separated from the ends of the first capillaries by respective gaps, or may include a single second capillary having one end disposed in the optical cell such that the end of the single second capillary is separated from the ends of the first capillaries. A sheath solution may be introduced into the optical cell to form sheath flows of the sheath solution around the ends of the first capillaries.

REFERENCES:
patent: 3764512 (1973-10-01), Greenwood et al.
patent: 4929329 (1990-05-01), Danby et al.
patent: 4938593 (1990-07-01), Morris et al.
patent: 5062942 (1991-11-01), Kambara et al.
patent: 5108179 (1992-04-01), Myers
L. Smith et al., "Fluorescence detection in automated DNA sequence analysis", Nature, vol. 321, Jun. 12, 1986, pp. 674-679.
Swerdlow et al., "Capillary gel electrophoresis for rapid, high resolution DNA sequencing", Nucleic Acids Research, vol. 18, No. 6, 1990, pp. 1415-1419.
H. Swerdlow et al., "Capillary gel electrophoresis for DNA sequencing", J. Chromatography, vol. 516, 1990, pp. 61-67.
Norman J. Dovichi et al, "Three DNA Sequencing Methods Using Capillary Gel Electrophoresis and Laser Induced Fluorescence" Analytical Chemistry, vol. 63, No. 24 (15 Dec. 1991) 2835-2841.
Yung-Fong Chen and Norman J. Dovichi, "Subattomole Amino Acid Analysis by Capillary Zone Electrophoresis and Laser-Induced Fluorescence" Science, vol. 242 (28 Oct. 1988) 362-365.
Yung-Fong Cheng and Norman J. Dovichi "Subattomole Amino Acid Analysis by Capillary Zone Electrophoresis and Laser-Induced Fluorescence" Science vol. 242 (1988) 562-564 No Month Available.
Norman J. Dovichi et al "Three DNA Sequencing Methods Using Capillary Gel Electrophoresis and Laser-Induced Fluorescence" Analytic Chemistry, vol. 63 No. 24 (1991) 2835-2841 No Month Available.
H. Kambara et al., "Optimization of Parameters in a DNA Sequenator Using Fluorescence Detection", Bio/Technology, vol. 6, Jul. 1988, pp. 816-821.
L. Morrison et al., "Solution-Phase Detection of Polynucleotides Using Interacting Fluorescent Labels and Competitive Hybridization", Analytical Biochemistry 183, pp. 231-232, 1989 No Month Available.

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