Assay of serine proteases using novel chromogenic enzyme substra

Details Assay of serine proteases using novel chromogenic enzyme substra Assay of serine proteases using novel chromogenic enzyme substra

1978-10-27

1980-07-22

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

G01N 3114

Patent

active

042140498

ABSTRACT:
Method for the quantitative determination of serine proteases which includes contacting the substance for which the determination is desired with a diagnostically active chromogenic substrate with good stability and high specificity to serine proteases having the formula: H--D--A.sub.1 --A.sub.2 --A.sub.3 --NH--R or salts thereof, wherein A.sub.1 is Gly, Ala, Val, Leu, Ile, Pip, Pro, or Aze; A.sub.2 is Gly, Ala, Val, Leu, Ile, Pip, Pro, Aze, or Phe; A.sub.3 is Arg, Lys, or Orn, and R is a chromophoric group.

REFERENCES:
patent: 3884896 (1975-05-01), Blomback et al.
patent: 4016042 (1977-05-01), Svendsen
patent: 4070245 (1978-01-01), Svendsen

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