Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1985-06-13
1988-04-19
Brown, J. R.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 28, 536 29, C07H 1512, C07H 1700
Patent
active
047390441
ABSTRACT:
A method for the preparation of a 3' end functionalized polynucleotide is disclosed. An amine-functionalized solid phase support is treated sequentially with an anhydride, then with an .omega.-hydroxylamine. A polynucleotide is chemically synthesized on the treated support and is subsequently cleaved therefrom by hydrolysis of the amide bonds. A polynucleotide having a 3' free primary amine is recovered for use in hybridization assays and other uses.
REFERENCES:
patent: 4310662 (1982-01-01), Crea
patent: 4415732 (1983-11-01), Caruthers et al.
patent: 4417046 (1983-11-01), Hsiung
Barker, et al., J. Biol. Chem., 22 7135-7147, (1972).
Bauman, et al., J. Histochem. Cytochem., 29, 227-237, (1981).
Broker, et al., Nucleic Acids Res., 5, 363-384, (1978).
Cuatrecasas, J. Biol. Chem., 245 (12), 3059-3065, (1970).
Kempe, et al., Nucleic Acids Res., 13, 45-57, (1985).
Manning, et al., Chromosoma (Berl), 53, 107-117, (1975).
Matteucci, et al., J. Am. Chem. Soc., 103, 3185-3191, (1981).
Richards, et al., Proc. Natl. Acad. Sci. (U.S.A.), 76, 676-680, (1979).
Suggs, et al., in Developmental Biology Using Purified Genes, Brown, et al. (eds.), Academic Press, New York, pp. 683-693, (1981).
Amgen
Brown J. R.
Rollins John W.
LandOfFree
Method for derivitization of polynucleotides does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for derivitization of polynucleotides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for derivitization of polynucleotides will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2176048