Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1986-03-31
1988-04-19
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435 68, 435 70, 435 71, 435 91, 4351721, 4351723, 4352402, 435243, 4352401, 4353171, 435320, 536 27, 935 9, 935 12, 935 29, 935 72, 935 73, C12N 120, C12N 1500, C12N 500, C12N 100, C12P 2100, C12P 2102, C12P 2104, C12P 1934, C07H 2104
Patent
active
047389281
ABSTRACT:
A method is provided for isolating and identifying a recombinant clone having a DNA segment therein coding for at least one desired heterologous polypeptide, at least a short amino acid sequence of which is known, by effecting cDNA synthesis on a mixture of mRNAs containing the mRNA coding of the desired polypeptide, isolating the resultant cDNA mixture, inserting the resultant cDNA into recombinant cloning vehicles, transforming hosts with the vehicles, separating the transformants and isolating and identifying a recombinant clone containing a DNA segment which is homologous over at least a portion thereof to at least one oligonucleotide probe specific for the DNA segment; wherein the probe is an extension of the nucleotide sequence of an oligonucleotide primer having a nucleotide sequence complementary to a region of the target mRNA coding for a portion of the known amino acid sequence, and is complementary to a longer region of the target mRNA coding for a longer portion of the known amino acid sequence. Recombinant clones coding for human histocompatibility antigens are provided, in particular, clones for HLA-B antigens.
REFERENCES:
patent: 4293652 (1981-10-01), Cohen
Sanger, et al., "DNA Sequencing with Chain-Terminating Inhibitors", Proc. Natl. Acad. Sci. U.S.A 74: 5463 (1977).
Houghton, et al., "The Amino-Terminal Sequence of Human Fibroblast Interferon as Deduced from Reverse Transcripts Obtained Using Synthetic Oligonucleotide Primers", Nucl. Acids. Res. 8: 1913 (1980).
Itakura, et al., "Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin", Science 198: 1056 (1977).
Ploegh, et al., "Molecular Cloning of a Human Histocompatibility Antigen cDNA Fragment", Proc. Natl. Acad. Sci. U.S.A 77: 6081 (1980).
Orr, et al., "Comparison of Amino Acid Sequence of Two Human Histocompatibility Antigens, HLA-A2 and HLA-B7: Location of Putative Alloantigenic Sites", Proc. Natl. Acad. Sci. U.S.A 76: 4395 (1979).
Noyes, et al., "Detection and Partial Sequence Analysis of Gastrin mRNA by Using an Oligodeoxynucleotide Probe", Proc. Natl. Acad. Sci. U.S.A 76: 1770 (1979).
Pereira Dennis
Sood Ashwani
Weissman Sherman M.
Martinell James
Yale University
LandOfFree
Method for cloning genes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for cloning genes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for cloning genes will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2175074