Method of optimizing a nucleic acid hybridization assay for dete

Details Method of optimizing a nucleic acid hybridization assay for dete Method of optimizing a nucleic acid hybridization assay for dete

1993-10-04

1996-02-06

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 78, C12Q 168

Patent

active

054895120

ABSTRACT:
A method for enhancing the hybridization signal of a nucleic acid hybridization assay for the DNA or RNA of a Salmonella in a sample by adding the sample to a RV growth medium under conditions sufficient to allow any Salmonella in the sample to propagate, propagating Salmonella, if any, in the medium for a time sufficient to allow the number of Salmonella to reach a predetermined titer, removing trace minerals from the medium, adding a nucleic acid probe to the medium under stringency conditions sufficient to allow the probe to preferentially hybridize with the Salmonella, if any, to form hybridization products that emit an enhanced signal, and assaying the medium to detect the enhanced signal. The method is especially suited for detecting Salmonella in food samples.

REFERENCES:
patent: 4689295 (1987-08-01), Taber et al.
Peterz, M. et al. (1989) Journal of Applied Bacteriology vol. 66, pp. 523-528.
Nucleic Acid Hybridization: A Practical Guide, B. D. Hames & S. J. Higgins, eds., 1985, IRL Press, Oxford, pp. 6-7.

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