Chemistry: physical processes – Physical processes – Crystallization
Patent
1980-07-14
1982-07-06
Turk, Arnold
Chemistry: physical processes
Physical processes
Crystallization
23905, 422 56, G01N 3372
Patent
active
043380956
ABSTRACT:
A method is described for the selective determination of the conjugated bilirubin (B.sub.c) or unconjugated bilirubin (B.sub.u), as well as the total bilirubin (B.sub.T), content of an aqueous liquid. The method comprises contacting the aqueous liquid with an analytical element having a dry reagent zone comprising an interactive mordant for bilirubin and thereafter detecting the absorption or emission spectra of the aqueous liquid at two or more wavelengths. The respective molar absorption or emission characteristics of B.sub.u and B.sub.c are predetermined for at least two of these wavelengths using a molecular weight of 584 for B.sub.u and a molecular weight in the range from about 750 to about 940 for B.sub.c. The B.sub.u, B.sub.c and/or B.sub.T content is determined from such spectra and absorption or emission characteristics. The interactive mordant for bilirubin is used to enhance the absorption characteristics or produce fluorescent emission of mordanted bilirubin.
REFERENCES:
patent: 4069017 (1978-01-01), Wu et al.
patent: 4204839 (1980-05-01), Wu et al.
Ostrow et al., "Isolation and Properties of Conjugated Bilirubin from Bile", Biochem. J. (1970) 120, 311-327.
Eastman Kodak Company
Hawley J. Jeffrey
Turk Arnold
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