Method for immunological assay using multilayer analysis sheet

Details Method for immunological assay using multilayer analysis sheet Method for immunological assay using multilayer analysis sheet

1980-09-29

1982-06-29

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

23915, 422 56, 424 12, G01N 3354, G01N 3358, G01N 3352

Patent

active

043370659

ABSTRACT:
In a photochemical immunological assay, an antigen or antibody is labelled with a spectral sensitizer or fogging agent for photographic use, and then an immune reaction is caused. The resulting reaction mixture is dropwise added to a multilayer analysis sheet. After either one of the labelled antigen or antibody (F) or the labelled antigen-antibody bound product (B) is brought into contact with silver halide by passing either one through a B/F separating layer provided in the multilayer analysis sheet, the analysis sheet is exposed with a light that the spectral sensitizer or fogging agent absorbs. Then, the resulting optical density obtained by development processing when a spectral sensitizer is used, or without development processing when a fogging agent is used is measured.
The multilayer analysis sheet comprises a support having provided thereon a silver halide layer and a B/F separating layer and makes the photochemical immunological assay simpler and more accurate.

REFERENCES:
patent: 4168146 (1979-09-01), Grubb

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