Chemistry: analytical and immunological testing – Involving production or treatment of antibody – Monoclonal antibody
Patent
1995-06-05
2000-12-12
Schwadron, Ronald B.
Chemistry: analytical and immunological testing
Involving production or treatment of antibody
Monoclonal antibody
435 7021, 435 701, 435325, 435330, 435344, 435440, 435452, 435455, 435465, 435354, 5303877, 5303888, 53038885, 530808, 530809, C12N 512, C12N 518, C12N 1508, G01N 3353
Patent
active
061597511
ABSTRACT:
This invention provides a method for preparing a hybridoma cell line which produces a monoclonal antibody which specifically recognizes and binds to a tumor associated antigen which comprises: (a) cotransfecting a CREF-Trans 6 cell line with DNA isolated from a neoplastic, human cell and a plasmid which encodes a selectable or identifiable trait; (b) selecting transfected cells which express the selectable or identifiable trait; (c) recovering the cells so selected in step (b); (d) injecting the cells so recovered in step (c) into a suitable marine host; (e) maintaining the resulting first murine host for a period of time effective to induce the cells injected in step (d) to form a tumor in the murine host; (f) isolating the tumor formed in step (e); (g) obtaining tumor cells from the isolated tumor in step (f); (h) coating the tumor cells obtained in step (9) with an antiserum generated against the CREF Trans-6 cell line (i) injecting the antiserum-coated cells from step (h) into a plurality of suitable second murine hosts; (j) screening the resulting second hosts from step (i) to identify hosts which produce serum reactive with the neoplastic human cell; (k) removing spleens from the second hosts so identified in step (j); (l) preparing from the spleens so removed in step (k) hybridomas; and (m) recovering therefrom a hybridoma cell line which produces a monoclonal antibody which specifically recognizes and binds to the tumor associated antigen.
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Schwadron Ronald B.
The Trustees of Columbia University in the City of New York
White John P.
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