Method of measuring biological ligand by the use of enzymes

Details Method of measuring biological ligand by the use of enzymes Method of measuring biological ligand by the use of enzymes

1984-11-13

1987-09-08

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 7, 435 18, 435 19, 435 22, 435 23, 436512, 436537, 436548, 436813, 436815, C12Q 134, C12Q 140, G01N 33536

Patent

active

046924043

ABSTRACT:
A method is provided which is highly sensitive and specific, and is suitable as a clinical test for the determination of physiological substances and trace components in humoral fluid. The method employs a conjugate of an enzyme capable of acting on a water-insoluble macromolecular substance and an antibody against the ligand (1) to be measured or a ligand (2) having an antigenic determinant common to one of the antigenic determinant(s) of the ligand (1). In the method of the invention, steric hindrance of the conjugate is emphasized by the enzyme reaction which is carried out at the boundary between solid phase and liquid phase.

REFERENCES:
patent: 4268663 (1981-05-01), Skold
patent: 4323647 (1982-04-01), Monji
I. Gibbons et al. Anal. Biochem. 102, 167-170, 1980.
R. H. Yolken, Rev. Infect. Diseases 4, 35-68, 1982.
Worthington Enzyme Manual, Worthington Biochemical Corporation, Freehold, N.J. 1972, pp. 63-64, 96-97, 132-133, 138-140.

Affiliated with

Also associated with

Rating

Method of measuring biological ligand by the use of enzymes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of measuring biological ligand by the use of enzymes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of measuring biological ligand by the use of enzymes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2158140