Method for immobilizing nucleic acids using modified capture pro

Details Method for immobilizing nucleic acids using modified capture pro Method for immobilizing nucleic acids using modified capture pro

1994-06-09

1997-06-17

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 536 243, 536 231, C12Q 168, C12P 1934, C07H 2104

Patent

active

056396090

ABSTRACT:
Method for immobilizing nucleic acids by hybridizing the nucleic acid with a capture probe. The capture probe is protected against enzymatic extension and/or enzymatic degradation of the formed hybrid.

REFERENCES:
patent: 5210015 (1993-05-01), Gelfand et al.
Iribarren et al., Proc. Natl. Acad. Sci. USA 87, 7747-7751 (1990).
Holland et al., Proc. Natl. Acad Sci USA 88, 7276-7280 (1991).
WO 91/03573, International Filing Date: Aug. 25, 1990 PCT/EP90/01423.
WO 91/15499, International Filing Date: Apr. 8, 1991 PCT/EP91/00665.
WO 91/02818, International Filing Date: Aug. 23, 1990 PCT/US90/04733.
WO 92/06216, International Filing Date: Oct. 4, 1991 PCT/EP91/01898.
WO 93/10267, International Filing date: Nov. 13, 1992 PCT/US92/09943.
"Enzymatic synthesis of biotin-labeled polynucleotides: Novel nucleic acid affinity probes" Biochemistry: Langer et al, Proc. Natl. Acad. Sci USA 78 (1981) pp. 6634-6637.

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