Method for assaying fatty acids

Details Method for assaying fatty acids Method for assaying fatty acids

1980-04-30

1982-09-14

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 15, 435 25, 435 26, 435 28, 435805, C12Q 128, C12Q 100, C12Q 148, C12Q 132

Patent

active

043496253

ABSTRACT:
There is disclosed a method for assaying fatty acids in a system containing albumin together with the fatty acids using acyl-CoA synthetase, characterized by performing the assay in the presence of a water-soluble salt of a dibasic fatty acid having 10 to 18 carbon atoms or a benzenesulfonate optionally having one or more C.sub.1 -C.sub.5 side chains.

REFERENCES:
patent: 4071413 (1978-01-01), Takahashi et al.
patent: 4301244 (1981-11-01), Kikuchi et al.
Zwierzykowski, et al., "Fractionation of fatty acids involving the use of surfactants and the selection of these for rape seed oil fatty acids as an example", Chem. Absts. vol. 81, No. 24, (1974), p. 150, Abs. #15496Fe.
Shimizu et al., "Enzymatic Microdetermination of Serum Free Fatty Acids", Anal. Biochem, vol. 98, No. 2, (1979), pp. 341-345.
Lagercrantz, et al., "Binding of Some Fatty Acids and Drugs to Immobilized Bovine Serum Albumin Studied by Affinity Chromatography" Anal. Biochem, vol. 99, (1979), pp. 352-364.
Meinertz, "Analytical Procedures for Free Fatty Acids", Progr. Biochem Pharmacol, vol. 6, (1971), pp. 317-367.

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